Expression of calpain-like proteins and effects of calpain inhibitors on the growth rate of Angomonas deanei wild type and aposymbiotic strains

Table 1 Possible Angomonas deanei calpain-like proteins recognized by the anti-calpain antibodies used in the present work.

Anti-calpain Antibody	Query protein accession #	Homologues in Angomonas deanei
Anti-calpain Antibody	Query protein accession #	Accession #	% Query cover	% Identity	E-value	Theoretical molecular mass (kDa)	Conserved domains ^a	PTM (number of sites) ^b
Anti-Dm-calpain	AHN56408.1	EPY32139.1	59	26	3e-39	80.2	cd00044	P(1)
		EPY32139.1	59	26	3e-39	80.2	cl07679	P(1)
		EPY35937.1	59	26	4e-39	80.3	cd00044	P(1)
		EPY35937.1	59	26	4e-39	80.3	cl07679	P(1)
		EPY35473.1	43	26	7e-31	81.0	cd00044	P(4) M(1)
		EPY35473.1	43	26	7e-31	81.0	pfam09149	P(4) M(1)
		EPY17791.1	29	31	1e-22	80.7	cd00044	P(1) M(1)
		EPY17791.1	29	31	1e-22	80.7	cl07679	P(1) M(1)
		EPY29841.1	37	25	3e-17	80.1	cd00044	P(1) M(1)
		EPY29841.1	37	25	3e-17	80.1	pfam09149	P(1) M(1)
		EPY33036.1	26	29	4e-22	47.6	cd00051	-
		EPY17550.1	9	33	1e-05	55.0	cd00051	M (1)
Anti-CAP5.5	AAG48626.1	EPY42129.1	45	40	6e-77	45.7	cd00051	P (1) M (1)
		EPY42129.1	45	40	6e-77	45.7	pfam09149	P (1) M (1)
		EPY33036.1	42	28	4e-47	47.6	cd00051	-
Anti-CDPIIb	AAM88579.1	EPY35937.1	49	31	1e-34	80.3	cd00044	-
		EPY35937.1	49	31	1e-34	80.3	cl07679	-
		EPY32139.1	49	31	1e-34	80.1	cd00044	-
		EPY32139.1	49	31	1e-34	80.1	cl07679	-
		EPY17791.1	57	28	1e-22	80.1	cd00044	P(1) M(1)
		EPY17791.1	57	28	1e-22	80.1	pfam09149	P(1) M(1)
		EPY35473.1	53	27	7e-25	81.0	cd00044	P(4)
		EPY35473.1	53	27	7e-25	81.0	pfam09149	P(4)
		EPY29841.1	19	29	1e-10	80.8	cd00044	P(1) M(1)
		EPY29841.1	19	29	1e-10	80.8	pfam09149	P(1) M(1)
		EPY36766.1	34	23	4e-05	62.0	cd00051	P(1)
		EPY36766.1	34	23	4e-05	62.0	pfam09149	P(1)
		EPY33036.1	30	28	4e-16	47.6	cd00051	-
		EPY42129.1	19	29	6e-11	45.7	cd00051	P(1) M(1)
		EPY42129.1	19	29	6e-11	45.7	pfam09149	P(1) M(1)
		EPY17550.1	12	38	5e-05	55.0	cd00051	P(6)

^aConserved calpain domains: cd00044 (or CysPc domain) corresponds to the domains IIa and IIb of the catalytic site of calpains; cd00051 (or EFh domain) is a EF-hand, calcium-binding motif present in calpains; pfam09149 and cl07679 (DUF1935 superfamily) are domains found in hypothetical proteins and calpains, with unknown function. ^bNumber of possible post-translacional modification sites (PTM) for palmitoylation (P) and myristoylation (M)

ISSN: 1471-2180