Faecal microbiota characterisation of horses using 16 rdna barcoded pyrosequencing, and carriage rate of clostridium difficile at hospital admission

Table 2 Detailed information on C. difficile positive horses, including molecular characterization and antibiotic resistance of the isolates

C. difficile positive horses								Isolates characterisation
Date of sampling	Animal identification	Age (years)	Diagnostic	Diarrhoea	Hospital stay (days)	Antibiotic treatment (days)^e	AINS treatment	PCR-ribotype	CE	tcdA tcdB	cdtA cdtB	tcdC ^a gyrA ^b MUT	PEN	CC	TE	VA MXF RA	LZE	XNL
15/10/2013	01	9	Wound and tendon injury	-	1	Pen-Gen-Xnl (1)^f	Dipyrone	UCL237	+	+	-	-	I	R	I	S	S	R
05/11/2013	03	16	Colic^c	-	1	-	Flunixin meglumine	UCL49	+	+	-	-	I	R	S	S	S	R
12/11/2013	04	4	Colic^d	-	2	-	-	014	+	+	-	-	I	S	S	S	S	R
03/12/2013	09	9	Multiple wounds	-	4	Pen-Gen (5)^f	Dipyrone	UCL36	-	-	-	-	I	R	S	S	R	R
09/12/2013	10	4	Proximal enteritis	-	4	-	Firocoxib Meloxidyl Dipyrone	UCL23f	+	+	-	-	I	R	S	S	S	R

MUT mutation
CE cytotoxicity assay using confluent monolayer MRC-5 cells
Gen gentamicin, Pen penicillin, CC clindamycin, TE tetracycline, VA vancomycin, MXF moxifloxacin, RA rifampicin, LZ metronidazole, E erythromycin, XNL ceftiofur
Iintermediate antimicrobial resistance
^aPresence of deletions in the regulator gene tcdC (118 bp-39 bp-17 bp)
^bPresence of mutation in the gyrA gene associated with moxifloxacin resistance
^cColic secondary to a gaseous distension of the caecum and a retraction of the colon
^dColic secondary to a pelvic flexure impaction (suspicion of a digestive tract verminosis causing weight loss)
^eTime before antibiotic administration in days ^fAntibiotic treatment in progress at the time of sampling

ISSN: 1471-2180