Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol

BMC Microbiology

Table 3 Primer pairs for each target gene for quantitative real-time PCR

Gene Name	Primer sequence (5′-3′)	Product size	Annealing temperature (°C)
Gene Name	Primer sequence (5′-3′)	(bp)	Annealing temperature (°C)
Up-regulated
AFLA_125090	AGGTTGTTCTCGGTCTGGTT	137	65
AFLA_125090	GCAAGGTCACCTACATGCAC	137	65
AFLA_075280	AGCTGGTTCGGTTTACCATC	128	64.5
AFLA_075280	ATGGCGATAGGGACAGGTAG	128	64.5
AFLA_057960	TGCAGACCAATGTTCATCCT	108	64.5
AFLA_057960	GTTGTCTCAGTCGTGCCAGT	108	64.5
AFLA_039390	CGCAACAAAGCAAGACATTT	128	61.4
AFLA_039390	GTCGGAGGGCTTGATTGTAT	128	61.4
AFLA_077590	CGTCGATTATGATGGAGACG	65	58.9
AFLA_077590	CACTGCTCAGCATTCCGTAT	65	58.9
AFLA_021650	GTTGGGCTATACGGAGGTGT	137	58.9
AFLA_021650	GCCATAGAGCAGCCAGTACA	137	58.9
AFLA_075300	GTGATTCATAGGGCCGACTT	156	64.5
AFLA_075300	GACGACAAGGTCTCCGGTAT	156	64.5
AFLA_023780	CTCCTCGACCGATTACCATT	125	57.1
AFLA_023780	CCCACTAACCACATCGACAG	125	57.1
Down-regulated
AFLA_117290	TTGCCCTCTGTTGAAGACTG	92	65
AFLA_117290	CCTCGTAGGACTTCCTCAGC	92	65
AFLA_112010	CAGTCGAACCCTATCCACCT	145	58.9
AFLA_112010	TACGTTCGGAGACACGGATA	145	58.9
AFLA_137320	AGCTACCCAGCACCAGAAGT	76	58.9
AFLA_137320	TGTAGGCAGGAGTCTGTTCG	76	58.9
AFLA_117420	CGGCAGAAGAGTACTGGTGA	106	57.1
AFLA_117420	ACGACTGGTGGTGACGATAA	106	57.1
AFLA_117340	TACAACCGGCTACAGCTCAC	113	57.1
AFLA_117340	TGATCGACTCGGAAAGACTG	113	57.1
AFLA_058990	ACGAGTCCTACCACCAGTCC	64	55
AFLA_058990	TTGATGGTTCCTCCTCCTTC	64	55
AFLA_128370	CCCTCTTTGGTAAGAATCGC	144	55.8
AFLA_128370	CGTCGGCCATATTCACATAG	144	55.8
AFLA_129450	GTACAGCCGTGGGATTCTTT	112	55.8
AFLA_129450	TGGAGCCAGTAGATATTGCG	112	55.8

ISSN: 1471-2180