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Fig. 2 | BMC Microbiology

Fig. 2

From: Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes

Fig. 2

Validation of the new heptaplex PCR assay. Lanes 1 and 8: 100 bp DNA ladder (NEB, UK); Lane 2: MRCoNS strain S. epidermidis NRS8 showing the markers cns, 16S, mecA, and tuf; Lane 3: Nottingham local MSCoNS showing the markers cns, 16S, and tuf; Lane 4: Group A Streptococcus showing only the 16S marker for bacterial 16SrRNA gene; Lanes 5 and 11: PCR negative control (Candida albicans); Lane 6: VRSA strain VRS1 showing the markers 16S, mecA, tuf, vanA, and spa; Lanes 7 and 14: mixed template comprising vancomycin-resistant S. aureus strain VRS1, methicillin susceptible CoNS strain S. lugdunensis NCTC12217 and PVL-positive MSSA strain NRS157 and showing all the seven markers (cns, 16S, mecA, tuf, pvl, vanA, and spa); Lane 9: PVL-negative MRSA strain Sanger252 showing the markers 16S, mecA, tuf, and spa; Lane 10: PVL-negative MSSA strain Sanger476 showing the markers 16S, tuf, and spa; Lane 12: PVL-positive MSSA strain NRS157 showing the markers 16S, tuf, pvl, and spa; Lane13: PVL-positive S. aureus strain USA400 (MW2) showing the markers 16S, mecA, tuf, pvl, and spa

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