Table 1 Primers, probes and cycling parameters
Name | Sequence | Function | Tm | Concentration |
|---|---|---|---|---|
ApF | TTTTGGGCGCTGAATACGAT | Forward Primer | 59 °C | 300 nM |
ApR | TCTCGAGGGAATGATCTAATAACGT | Reverse Primer | 58 °C | 300 nM |
ApM | TGCCTGAAC AAGTTATG | 5’ hydrolysis probe | 69 °C | 300 nM |