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Fig. 5 | BMC Microbiology

Fig. 5

From: Penicillium chrysogenum as a model system for studying cellular effects of methylglyoxal

Fig. 5

Analysis of changes of protein abundance by methylglyoxal treatment in Ws54-1255 by isoelectric focusing/2D-SDS-PAGE/peptide mass fingerprinting. a Separated proteins of an untreated Ws54-1255 culture grown in YGG medium for 2.5 days at 25 °C visualized by Coomassie Blue staining. Proteins with different abundance compared to the culture grown in the presence of 0.2 % (v/v) methylglyoxal are marked by black circles. The identity of selected peptide spots was determined by peptide mass fingerprinting: R-peptide belonging to 60S ribosomal protein L5 (Pc13g11570); 1-peptide belonging to aldo-keto reductase (Pc22g04850); 2-peptide belonging to 5-methyl-tetrahydropteroyl-triglutamate-homocysteine S-methyltransferase (Pc22g18630); 3-peptide belonging to NAD-dependent formate dehydrogenase (Pc12g04310). b Separated proteins of a Ws54-1255 culture grown in YGG medium for 2.5 days at 25 °C in the presence of 0.2 % (v/v) methylglyoxal visualized by Coomassie Blue staining. Regions corresponding to the gel shown in panel a are marked with dotted circles. pI: isoelectric point, MG: methylglyoxal, Mr: relative molecular mass

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