Fig. 2
From: Penicillium chrysogenum as a model system for studying cellular effects of methylglyoxal
Localisation of GFP-SKL in Ws54-1255 (GFP-SKL) treated with methylglyoxal and/or rapamycin. At the indicated times cultures grown on starvation pads supplemented with or without methylglyoxal and/or rapamycin were analysed using fluorescence microscopy. Representative images are shown for each time point. a control; b + 0.05 % (v/v) methylglyoxal; c + 1 μM rapamycin; d + 0.05 % (v/v) methylglyoxal/+ 1 μM rapamycin. Corresponding bright field areas are shown below each fluorescence channel image. White arrows: GFP-SKL localised to vacuoles. Scale bars: 10 μm