Table 2 Primers used in this study
Name | Sequence 5′ – 3′ | Orientation/Restriction site |
|---|---|---|
hp377I | GGCGATACTTACCAGCAAG | Fwd/Ø |
hp377II | ATCCACTTTTCAATCTATATCTGTCTATATTGTCTCGCTCATC | Rev/Ø |
hp377IIa | CAAGGCAAT CTGCCTCCTCATGTCTATATTGTCTCGCTCATC | Rev/Ø |
hp377IIIa | GGATGAATTGTTTTAGTACCAATTTATCGGCGATGGGAAG | Fwd/Ø |
hp377IV | GTTTGGTGGTGTCATTAAGAGTG | Rev/Ø |
hp377ex1 | GAGGCCATGGGCAAATCCAACAATAAAGAC | Fwd/NcoI |
hp377ex2 | GTGCTCGAGGTTAGACTTGCTTTTAGAAAG | Rev/XhoI |
HP0377zew1 | CTAAGGTGCGAGTAATAGAG | Fwd/Ø |
HP0377zew2 | CTATGCCTTCATTCCTGTTG | Rev/Ø |
hp0377mutF | GAGGCATATGATGTTTTCACTTTCTTATGTTTCC | Fwd/NdeI |
hp0377mutR | GAGGATCCCTAGTTAGACTTGCTTTTAGAAAG | Rev/BamHI |
N6_377_C89S_for | CTTTCGCAATAGGAGCTACCATTACGCCCAAAAACTAAAA | Fwd/Ø |
N6_377_C89S_rev | CTTTCGCAATAGGAGCTACCATTACGCCCAAAAACTAAAA | Rev/Ø |
hp377_C92SII | GTTTTTGGCCGTAATGGTTGCTCCTATTCCGAAAG | Fwd/Ø |
hp377_C92SII_rev | CTTTCGGAATAGGAGCAACCATTACGGCCAAAAAC | Rev/Ø |
hp377_C25A_for | ATTGATTTCGCTGTTTTTAAGCGCTGCCAAATCCAACAAT AAAGACAAGTTA | Fwd/Ø |
hp377_C25A_rev | TAACTTGTCTTTATTGTTGGATTTGGCAGCGCTTAAAAAC AGCGAAATCAAT | Rev/Ø |
N6_HP0264_fwd1 | GATAGTAAGGGCGTGAGG | Fwd/Ø |
N6_HP0265_up_Bam | AAGGGATCCGCGATTAAAGAGAGCTT | Fwd/BamHI |
N6_HP0265_dw_Sal | TTAAGCGTAGCGATGTCGACTAGTTG | Rev/SalI |
N6_HP0265_rev1 | GGCGTAATGGCTGATGAG | Rev/Ø |
N6_HP0265_up_rev | TAGCTGCAGCCCGGGTAGCCACCACAAACATCAAGGG | Rev/Ø |
N6_HP0265_dw_fwd | CTACCCGGGCTGCAGCTATCCTTATGGTGGTGTTTG | Fwd/Ø |
KM1f | TGAGGAGGCAGATTGCCTTG | Fwd/Ø |
KM2r | GGTACTAAAACAATTCATCC | Rev/Ø |
1hp265F-6HisNew | GAGCCATGGCACACCACCACCACCACCACATGTTTGATAACACGCTTG | Fwd/NcoI |
2hp265RPstI | GTGCTGCAGCTATTTTTGCAAGAAATTCGTCAGAC | Rev/PstI |
Hp1227_NdeI-for | GCGCATATGACCGATGTTAAAGCCCTTG | Fwd/NdeI |
Hp1227_XhoI-rev | CGCTCGAGTTTGAGGGTGGGGATGTAT | Rev/XhoI |