Larva of the greater wax moth, Galleria mellonella , is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum

Stuart McMillan; David Verner-Jeffreys; Jason Weeks; Brian Austin; Andrew P. Desbois

doi:10.1186/s12866-015-0466-9

Table 1 Vibrio anguillarum isolates and strains used in this study

From: Larva of the greater wax moth, Galleria mellonella, is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum

Isolate/strain^a	Genotype	Culture medium^b	Reference
DM16	NB10 derivative carrying an in-frame 3’-end deletion in flaA	TSB/TSA + 1.5%NaCl	[27]
JR1	STR^r, CHL^r M93Sm derivative carrying an inactivating insertion in vah1	LB agar/broth + 1%NaCl	[28]
KD12	NB10 derivative carrying an in-frame 5’-end deletion in flaD	TSB/TSA + 1.5%NaCl	[29]
KD27	NB10 derivative carrying an in-frame 5’-end deletion in flaE	TSB/TSA + 1.5%NaCl	[29]
M93Sm	Plasmid deficient; spontaneous STR^r mutant of wild type M93 isolated from Plecoglossus altivelis	LB agar/broth + 1%NaCl	[41]
NB10	Wild type	TSB/TSA + 1.5%NaCl	[42]
NB10 cured	NB10 cured of pJM1 virulence plasmid	TSB/TSA + 1.5%NaCl	[32]
NB12	CHL^r NB10 derivative carrying a inactivating insertion in empA	TSB/TSA + 1.5%NaCl	[30]
S123	STR^r, CHL^r M93Sm derivative carrying a inactivating insertion in rtxA	LB agar/broth + 1%NaCl	[7]
S183	STR^r, CHL^r, KAN^r M93Sm derivative double mutant carrying an inactivating insertions in rtxA and in-frame deletion in vah1	LB agar/broth + 1%NaCl	[7]
Vib1 (= 6018/1 = ATCC 43305)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib39 (= 178/90)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib44 (= 261/91)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib56 (= 601/91)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib64 (= A023)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib79 (= LMG 12101)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib85 (= 87-9-117)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib87 (= T265)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib88 (= 51/82/2)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib93 (= 850610-1/6a)	Wild type	TSB/TSA + 1.5%NaCl	[21]
Vib134 (= 91-8-178)	Wild type	TSB/TSA + 1.5%NaCl	[21]

TSA, tryptone soya agar; TSB, tryptone soya broth; CHL^r, chloramphicol-resistant; KAN^r, kanamycin-resistant; STR^r, streptomycin-resistant
^aStrain numbers in brackets refer to the nomenclature used by Pedersen et al. [25]
^bCHL, KAN and STR were added to the medium at the concentrations described in the Materials and Methods for reviving cryopreserved bacteria and preparing inocula for injection

Back to article page