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Table 1 Vibrio anguillarum isolates and strains used in this study

From: Larva of the greater wax moth, Galleria mellonella, is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum

Isolate/straina

Genotype

Culture mediumb

Reference

DM16

NB10 derivative carrying an in-frame 3’-end deletion in flaA

TSB/TSA + 1.5%NaCl

[27]

JR1

STRr, CHLr M93Sm derivative carrying an inactivating insertion in vah1

LB agar/broth + 1%NaCl

[28]

KD12

NB10 derivative carrying an in-frame 5’-end deletion in flaD

TSB/TSA + 1.5%NaCl

[29]

KD27

NB10 derivative carrying an in-frame 5’-end deletion in flaE

TSB/TSA + 1.5%NaCl

[29]

M93Sm

Plasmid deficient; spontaneous STRr mutant of wild type M93 isolated from Plecoglossus altivelis

LB agar/broth + 1%NaCl

[41]

NB10

Wild type

TSB/TSA + 1.5%NaCl

[42]

NB10 cured

NB10 cured of pJM1 virulence plasmid

TSB/TSA + 1.5%NaCl

[32]

NB12

CHLr NB10 derivative carrying a inactivating insertion in empA

TSB/TSA + 1.5%NaCl

[30]

S123

STRr, CHLr M93Sm derivative carrying a inactivating insertion in rtxA

LB agar/broth + 1%NaCl

[7]

S183

STRr, CHLr, KANr M93Sm derivative double mutant carrying an inactivating insertions in rtxA and in-frame deletion in vah1

LB agar/broth + 1%NaCl

[7]

Vib1 (= 6018/1 = ATCC 43305)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib39 (= 178/90)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib44 (= 261/91)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib56 (= 601/91)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib64 (= A023)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib79 (= LMG 12101)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib85 (= 87-9-117)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib87 (= T265)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib88 (= 51/82/2)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib93 (= 850610-1/6a)

Wild type

TSB/TSA + 1.5%NaCl

[21]

Vib134 (= 91-8-178)

Wild type

TSB/TSA + 1.5%NaCl

[21]

  1. TSA, tryptone soya agar; TSB, tryptone soya broth; CHLr, chloramphicol-resistant; KANr, kanamycin-resistant; STRr, streptomycin-resistant
  2. aStrain numbers in brackets refer to the nomenclature used by Pedersen et al. [25]
  3. bCHL, KAN and STR were added to the medium at the concentrations described in the Materials and Methods for reviving cryopreserved bacteria and preparing inocula for injection