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Fig. 3 | BMC Microbiology

Fig. 3

From: Larva of the greater wax moth, Galleria mellonella, is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum

Fig. 3

Replication of 11 wild-type Vibrio anguillarum isolates in the haemolymph of Galleria mellonella larvae during 120 h after injection at 0 h with 1 × 105 CFU. Please note that the CFU/mL value at 0 h refers to the PBS inoculum whereas at the other sampling times the CFU/mL value refers to the haemolymph. It was not possible to obtain data for all strains at each sampling time after 48 h because most larvae were already dead and so were not sampled. For comparative purposes, the geometric mean (and standard error) of CFU/mL in haemolymph for Vib1 at 2 h, 4 h, 8 h, 24 h and 48 h was 6.86 (0.10), 6.99 (0.07), 7.40 (0.17), 8.70 (0.15) and 8.64 (0.85), respectively. For clarity, the unmanipulated and uninfected control groups data are not shown. Data points indicate geometric mean; error bars have not be added. n = 4

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