Skip to main content

Table 2 Kinetic parameters of AbGSTs in enzymatic assays

From: Characterization of glutathione transferases involved in the pathogenicity of Alternaria brassicicola

  AbGTT1.2 AbGSTO1 AbUre2pB1 AbGSTFuA1
K m (mM)     
CDNB 1.82 ± 0.33 ND 5.01 ± 0.40 0.40 ± 0.02
Al-ITC ND ND ND 0.12 ± 0.02
Bz-ITC ND ND ND 0.18 ± 0.03
Ph-ITC ND ND ND 0.11 ± 0.02
Cu-OOH 0.12 ± 0.04 ND 0.77 ± 0.06 ND
β-ME-SG ND 1.12 ± 0.02 ND ND
GSH 1.33 ± 0.10 0.45 ± 0.13 0.87 ± 0.02 0.50 ± 0.0004
k cat (s -1 )     
CDNB 0.10 ± 0.05 ND 1.50 ± 0.08 38.18 ± 6.10
Al-ITC ND ND ND 76.80 ± 11.64
Bz-ITC ND ND ND 11.81 ± 1.82
Ph-ITC ND ND ND 20.54 ± 2.96
Cu-OOH 6.82 ± 1.18 ND 0.20 ± 0.01 ND
β-ME-SG ND 49.00 ± 9.40 ND ND
  1. The apparent K m values for all compounds were determined using a 0.1-50 mM concentration range in the presence of 3 mM GSH. The K m value for GSH was determined with 2 mM CDNB and a 0.01 to 10 mM GSH concentration range. The apparent K m and k cat values were calculated by nonlinear regression using the Michaelis-Menten equation (r2 > 0.99). Data are represented as mean ± S.D. (n ± 3)
  2. ND Not detectable