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Table 3 Stability of a recombinant plasmid, pKZ200, and plasmids, pBCNF5603 and pCP13, in C. perfringens strains

From: Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603

Incidence of colonies carrying a recombinant plasmid, pKZ200, in ATCC3624 strain
   Rate of Cp resistant colonies*
clone 1 Culture with Cp 92 %
  Culture without Cp 40 %
clone 2 Culture with Cp 84 %
  Culture without Cp 44 %
Incidence of colonies carrying a ecombinant plasmid, pKZ200, in strain 13
   Rate of Cp resistant colonies*
clone 1 Culture with Cp 80 %
  Culture without Cp 40 %
clone 2 Culture with Cp 96 %
  Culture without Cp 44 %
Incidence of colonies carrying a bacteriosin gene (bcn5603)-encoding plasmid, pBCNF5603, in F5603 strain
   Rate of PCR positive colonies*, #
clone 1 PCR for the rep gene 100 %
  PCR for the bcn5603 gene 100 %
clone 2 PCR for the rep gene 100 %
  PCR for the bcn5603 gene 100 %
Incidence of colonies carrying the beta2 toxin gene-encoding plasmid, pCP13, in strain 13
   Rate of PCR positive colonies*, #
clone 1 PCR for the PCP63 gene 100 %
  PCR for the cpb2 gene 100 %
clone 2 PCR for the PCP63 gene 100 %
  PCR for the cpb2 gene 100 %
  1. Culture samples with or without 10 µg/ml chloramphenicol were collected after ~60 generations
  2. *: Rate of randomly selected colonies
  3. #: To investigate the presence of pBCNF5603 in F5603 strain or pCP13 in strain13, randomly selected colonies were tested with colony PCR assay for three genes, rep (PCP63), bcn5603, and cpb2 genes. Colony PCR reactions were performed under the same conditions as described in the plasmid compatibility section