Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603

Table 3 Stability of a recombinant plasmid, pKZ200, and plasmids, pBCNF5603 and pCP13, in C. perfringens strains

Incidence of colonies carrying a recombinant plasmid, pKZ200, in ATCC3624 strain
		Rate of Cp resistant colonies^*
clone 1	Culture with Cp	92 %
	Culture without Cp	40 %
clone 2	Culture with Cp	84 %
	Culture without Cp	44 %
Incidence of colonies carrying a ecombinant plasmid, pKZ200, in strain 13
		Rate of Cp resistant colonies^*
clone 1	Culture with Cp	80 %
	Culture without Cp	40 %
clone 2	Culture with Cp	96 %
	Culture without Cp	44 %
Incidence of colonies carrying a bacteriosin gene (bcn5603)-encoding plasmid, pBCNF5603, in F5603 strain
		Rate of PCR positive colonies^{*, #}
clone 1	PCR for the rep gene	100 %
	PCR for the bcn5603 gene	100 %
clone 2	PCR for the rep gene	100 %
	PCR for the bcn5603 gene	100 %
Incidence of colonies carrying the beta2 toxin gene-encoding plasmid, pCP13, in strain 13
		Rate of PCR positive colonies^{*, #}
clone 1	PCR for the PCP63 gene	100 %
	PCR for the cpb2 gene	100 %
clone 2	PCR for the PCP63 gene	100 %
	PCR for the cpb2 gene	100 %

Culture samples with or without 10 µg/ml chloramphenicol were collected after ~60 generations
^*: Rate of randomly selected colonies
^#: To investigate the presence of pBCNF5603 in F5603 strain or pCP13 in strain13, randomly selected colonies were tested with colony PCR assay for three genes, rep (PCP63), bcn5603, and cpb2 genes. Colony PCR reactions were performed under the same conditions as described in the plasmid compatibility section

ISSN: 1471-2180