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Table 1 Strains and plasmids used in this study

From: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Strain or plasmid Relevant characteristic Reference
C. glutamicum   
WT wild type strain ATCC 13032 [9]
ΔphoRS deletion of the phoRS operon encoding the two component system PhoRS [33]
ΔramB Deletion of ramB encoding regulator of acetate metabolism B [41]
E. coli   
BL21(DE3) ompT hsdS B(rBmB) gal dcm (DE3) [64]
DH5α F thi-1 endA1 hsdr17(r, m) supE44 ΔblacU169 (ф80lacZΔM15) recA1 gyrA96 relA1 [65]
Plasmids   
pGEM-T cloning vector Promega, WI, USA
pET2 promoter-probe vector [54]
pET2-RF0 pET2 with pstSCAB promoter fragment RF0 This study
pET2-R0F0 pET2 with pstSCAB promoterfragment R0F0 This study
pET2-R1F0 pET2 with pstSCAB promoter fragment R1F0 This study
pET2-R2F0 pET2 with pstSCAB promoter fragment R2F0 This study
pET2-R3F0 pET2 with pstSCAB promoter fragment R3F0 This study
pET2-R0F1 pET2 with pstSCAB promoter fragment R0F1 This study
pET2-R0F2 pET2 with pstSCAB promoter fragment R0F2 This study
pET2-R0F3 pET2 with pstSCAB promoter fragment R0F3 This study
pET2-RcFc pET2 with pstSCAB promoter fragment RcFc This study
pET2-RcFm pET2 with pstSCAB promoter fragment RcFm This study
pET2-RmFc pET2 with pstSCAB promoter fragment RmFc This study
pET2-RmFm pET2 with pstSCAB promoter fragment RmFm This study
pET29-ramB-his KanR; pET29-Histag derivative for over production of RamB with a C-terminal histidine tag [41]