Skip to main content

Table 1 Sterols obtained from S. cerevisiae strains used in this study (mg per g of dry yeast)

From: Identification and functional characterization of the CYP51 gene from the yeast Xanthophyllomyces dendrorhous that is involved in ergosterol biosynthesis

 

S. cerevisiae strains

 

(Sc- erg 11 +/− )

Sc-h ERG11

Sc-h CYP51

Cultivation Time (h)

10

24

56

10

24

56

10

24

56

Total sterols

25 ± 5

11 ± 1

21 ± 3

21 ± 8

16 ± 5

38 ± 7

19 ± 3

9 ± 1

20 ± 2

Ergosterol (11 min)

23 ± 5

10 ± 1

20 ± 2

17 ± 2

15 ± 4

34 ± 6

16 ± 3

9 ± 3

18 ± 2

Peak 2 (12.5 min)

ND

0.03 ± 0.02

0.2 ± 0.3

ND

0.06 ± 0.02

0.4 ± 0.4

ND

ND

0.21 ± 0.04

Peak 3 (15 min)

3 ± 1

0.7 ± 0.1

1.45 ± 0.03

4 ± 1

1.5 ± 0.4

3.1 ± 0.4

2.4 ± 0.6

0.7 ± 0.1

1.6 ± 0.1

  1. ND: Not detected. Table values correspond to the average result from three independent cultures ± standard deviations. The RP-HPLC retention time is indicated in parenthesis.