RT-qPCR analysis of the HMGR, crtR and CYP51 genes in the wild-type and CBS-CYP51
+/− strains. The HMGR
(B) and CYP51
(C) transcript levels were determined by RT-qPCR after 24 and 48 h of cultivation of the wild-type and the CBS-CYP51
+/−strains. Each transcript level was normalized with respect to the transcript level of the actin gene and then with respect to the wild-type strain: CBS 6938 (=1, black bars) and CBS-CYP51+/− (gray bars). Values are the mean ± standard error of three independent experiments (* p ≤ 0.05; Student’s t test).