Laser scanning confocal microscope (LSCM) and transmission electron microscopy/energy dispersive X-ray spectroscopy (TEM/EDS) analysis of strain S12 cells. (a) and (b): LSCM images of cells after biodecolorization for 12 h with 60 mM Fe0. The red point represents dead cells, and the green point represents live cells. The iron particles are indicated in black. (c): LSCM image of cells after biodecolorization without Fe0. (d) and (e): TEM images and EDS profiles of cells after biodecolorization for 12 h with 60 mM Fe0, showing the presence of iron precipitates inside and outside the cell membrane. (f): TEM images and EDS profiles of cells after biodecolorization without Fe0. The white cross is the site of EDS analysis. The white short line is a scale bar, and the corresponding length is on the top of the scale bar.