Figure 6

Role of AcsA, AcsS, and AcsR in V. vulnificus growth using glucose or acetate as a carbon source. A - Role of acsA in bacterial growth in the presence of glucose or acetate as a carbon source. Wild-type and ΔacsA strains were inoculated in the minimal medium containing glucose (a) or acetate (b), and then bacterial growth was monitored using a spectrophotometer (OD₅₉₅). To confirm the growth defect of the mutant was caused by a deletion of the acsA gene, the ΔacsA mutant supplied with a broad-host-range vector containing the original acsA gene (pRKacsA) was grown in an acetate-minimal medium supplemented with 3 μg/ml tetracycline (c). For comparison, wild type and ΔacsA carrying the vector plasmid (pRK415) were included in this assay; B – Growth of wild-type and ΔacsR strains; C – Growth of wild-type and ΔacsS strains; V. vulnificus strains were inoculated to the minimal medium containing glucose (a) or acetate (b), and then bacterial growth was monitored using a spectrophotometer (OD₅₉₅). To confirm the growth defect of each mutant was caused by a deletion of the acsR or acsS gene, each mutant supplied with a broad-host-range vector containing the original acsR or acsS genes (pRKacsR or pRKacsS, respectively) was grown in acetate-minimal medium supplemented with 3 μg/ml tetracycline (c). For comparison, wild type and mutants carrying the vector plasmid (pRK415) were also included in this assay; D – Complementation of ΔacsR (a) and ΔacsS (b) mutant V. vulnificus with a broad-host-range vector containing the original acsA gene (pRKacsA) and their growth in acetate minimal medium.