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Table 1 Primers used in this study

From: Heterologous expression and processing of the flavescence dorée phytoplasma variable membrane protein VmpA in Spiroplasma citri

Primers Sequences Target gene Positions Accession numbers
VAF1 5′-ATGAT GGATCCATTACAGATTTGAGTGGTGT-3′ vmpA* 765-784 LN680870
VAF3 5′-ATGAT GGATCCGTTTAACTAATATAAGTTAAACTCTA-3′ vmpA* 242-267 LN680870
VAR2 5′-TCATA AGATCTCAAAATAAATCAATAAAAAACTCAC-3′ vmpA* 1874-1896 LN680870
BlaF1 5′-ATGATGGATCCGCTCACCCAGAAACGCTGGTG-3′ bla** 2918-2938 VB0044
BlaR2 5′-ATGATAGATCTCAAGCAGCAGATTACGCGCAG-3′ bla** 1937-1957 VB0044
  1. *vmpA gene of the FD92 phytoplasma.
  2. **β-lactamase gene from phagemid Bluescribe M13 plus (pBS+).
  3. Bold characters indicate endonuclease restriction sites used for cloning.