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Table 1 Primers used in this study

From: Heterologous expression and processing of the flavescence dorée phytoplasma variable membrane protein VmpA in Spiroplasma citri

Primers

Sequences

Target gene

Positions

Accession numbers

VAF1

5′-ATGAT GGATCCATTACAGATTTGAGTGGTGT-3′

vmpA*

765-784

LN680870

VAF3

5′-ATGAT GGATCCGTTTAACTAATATAAGTTAAACTCTA-3′

vmpA*

242-267

LN680870

VAR2

5′-TCATA AGATCTCAAAATAAATCAATAAAAAACTCAC-3′

vmpA*

1874-1896

LN680870

BlaF1

5′-ATGATGGATCCGCTCACCCAGAAACGCTGGTG-3′

bla**

2918-2938

VB0044

BlaR2

5′-ATGATAGATCTCAAGCAGCAGATTACGCGCAG-3′

bla**

1937-1957

VB0044

  1. *vmpA gene of the FD92 phytoplasma.
  2. **β-lactamase gene from phagemid Bluescribe M13 plus (pBS+).
  3. Bold characters indicate endonuclease restriction sites used for cloning.
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BMC Microbiology

ISSN: 1471-2180

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