The BB0028 mutant exhibits impaired growth
A. Growth rate analysis of the parental strain (■) and BB0028 mutant strain (▲). The BB0028 mutant required 3–4 more days to reach stationary phase than the parental strain. The inset confirms by immunoblot that BB0028 was not expressed by the BB0028 mutant (▲) during culture. As a control, the wildtype, parental strain (■) was also immunoblotted for BB0028. Lysates also were probed with FlaB specific antibodies to confirm equivalent loading. B. Growth rate comparison of the wildtype (■) and BamD/BB0324 mutant (▲) strains.