Figure 3
From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
In vivo macrocolony biofilm development of strains carrying DegU protein variants. (A) Macrocolony morphology of 3610 (wild-type strain), degS (NRS1358), degU (NRS1314), degU + P hyspank -degU (H12L)-lacI (NRS1325) and ΔdegSU + P hyspank -degU (H12L)-lacI (NRS4419) grown on MSgg agar plates in the absence (−) or presence (+) of 50 μM IPTG. (B) Colony morphology of 3610 (wild-type strain), degU + P hy-spank -degU (H12L, D56N)-lacI (NRS4762) and ΔdegSU + P hy-spank -degU (H12L, D56N)-lacI (NRS4761) grown on MSgg agar plates in the absence (−) or presence (+) of 50 μM IPTG. All colonies were imaged after 48 h incubation at 30°C.