Figure 2
From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
Analysis of exoprotease production by strains expressing DegU protein variants. (A) Exoprotease production of 3610 (wild type strain), degS (NRS1358), degU (NRS1314), degU + P hyspank -degU (H12L)-lacI (NRS1325) and ΔdegSU + P hyspank -degU (H12L)-lacI (NRS4419) grown on LB agar plates containing 1.5% milk in the absence (−) or presence (+) of 50 μM IPTG. (B) Exoprotease production of 3610 (wild type strain), degU + P hy-spank -degU (H12L, D56N)-lacI (NRS4762) and ΔdegSU + P hy-spank -(H12L, D56N)-lacI (NRS4761) grown on LB agar plates containing 1.5% milk in the absence (−) or presence (+) of 50 μM IPTG. Plates were photographed after 16 h incubation at 37°C.