A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition

BMC Microbiology

Table 1 Plasmids and strains used in the study

Strains or plasmids	Relevant characteristics	Reference/source
P. fluorescens
MFE01	Air isolate, Rif^R	[31]
MFE01∆hcp2	MFE01 with early stop codon in hcp2	[31]
MFE01∆hcp1	MFE01 with hcp1 disruption	This study
MFE01∆hcp1 + hcp1	MFE01∆hcp1 with pPSV35 carrying the wild-type hcp1 gene	This study
MFE01∆hcp1∆hcp2	MFE01 with hcp1 and hcp2 mutations	This study
MFE01∆tssC	MFE01 with tssC disruption	This study
MFN1032	Clinical strain	[33]
MFP05	Skin isolate	[32]
Pf-5	Plant isolate	[34]
MFE07	Air isolate	This study
Escherichia coli
DH5αmcr	General cloning strain	LMSM collection
S17.1	RP4-2-Tc::Mu, aph::Tn7, recA, SmR, donor strain for conjugation	[48]
Vectors
pPSV35	P. aeruginosa oriV, lacIq mob+, PlacUV5, pUC18 MCS, expression vector, Gm^R	[49]
pSMC21-gfp	Replicative plasmid, KmR, gfp	[46]
pME3087	Suicide plasmid, Tc^R	[47]
pUC19	Replicative plasmid, lacZ, Ap^R	Invitrogen®

ISSN: 1471-2180