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Table 1 Bacterial strains and plasmids used in this study

From: The autoinducer synthases LuxI and AinS are responsible for temperature-dependent AHL production in the fish pathogen Aliivibrio salmonicida

Bacterial strain or plasmid Description a Source or reference
A. salmonicida
  LFI1238 Wild type, isolated from Atlantic cod [11]
  ΔlitR LFI1238 containing an in-frame deletion of litR [10]
  ΔainS LFI1238 containing an in-frame deletion of ainS This study
  ΔainS/luxI ˉ ΔainS with insertional disruption of luxI; Cmr This study
  luxI ˉ LFI1238 with insertional disruption of luxI; Cmr This study
  luxR1ˉ LFI1238 with insertional disruption of luxR1 gene, Ermr This study
  luxR2ˉ LFI1238 with insertional disruption of luxR2 gene, Ermr This study
E. coli
  S17-1 Donor strain for conjugation, λ-pir [42]
  DH5αλ pir Donor strain for conjugation [45]
  DH5α Helper strain for conjugation Invitrogen
Plasmids
  pDM4 Cmr; suicide vector with an R6K origin (λ-pir requiring) and sacBR [43]
  pNQ705 Cmr; suicide vector with an R6K origin (λ-pir requiring) [43]
  pEVS122 Ermr; suicide vector with an R6Kγ oriV, oriT RP4 , lacZα,cosN, loxP, incD [45]
  pEVS104 Helper plasmid [44]
  pDM4ΔainS pDM4 containing a fragment of ainS harbouring an internal deletion This study
  pNQ705luxI pNQ705 containing a 245 bp fragment of luxI This study
  pEVSluxR1 pEVS122 containing a 224 bp fragment of luxR1 This study
  pEVSluxR2 pEVS122 containing a 280 bp fragment of luxR2 This study
  1. aCmr, Chloremphenicol resistance gene. Ermr, Erythromycin resistance gene.