The autoinducer synthases LuxI and AinS are responsible for temperature-dependent AHL production in the fish pathogen Aliivibrio salmonicida

Table 1 Bacterial strains and plasmids used in this study

Bacterial strain or plasmid		Description ^a	Source or reference
A. salmonicida
	LFI1238	Wild type, isolated from Atlantic cod	[11]
	ΔlitR	LFI1238 containing an in-frame deletion of litR	[10]
	ΔainS	LFI1238 containing an in-frame deletion of ainS	This study
	ΔainS/luxI ˉ	ΔainS with insertional disruption of luxI; Cm^r	This study
	luxI ˉ	LFI1238 with insertional disruption of luxI; Cm^r	This study
	luxR1ˉ	LFI1238 with insertional disruption of luxR1 gene, Erm^r	This study
	luxR2ˉ	LFI1238 with insertional disruption of luxR2 gene, Erm^r	This study
E. coli
	S17-1	Donor strain for conjugation, λ-pir	[42]
	DH5αλ pir	Donor strain for conjugation	[45]
	DH5α	Helper strain for conjugation	Invitrogen
Plasmids
	pDM4	Cm^r; suicide vector with an R6K origin (λ-pir requiring) and sacBR	[43]
	pNQ705	Cm^r; suicide vector with an R6K origin (λ-pir requiring)	[43]
	pEVS122	Erm^r; suicide vector with an R6Kγ oriV, oriT _RP4, lacZα,cosN, loxP, incD	[45]
	pEVS104	Helper plasmid	[44]
	pDM4ΔainS	pDM4 containing a fragment of ainS harbouring an internal deletion	This study
	pNQ705luxI	pNQ705 containing a 245 bp fragment of luxI	This study
	pEVSluxR1	pEVS122 containing a 224 bp fragment of luxR1	This study
	pEVSluxR2	pEVS122 containing a 280 bp fragment of luxR2	This study

ISSN: 1471-2180