A. salmonicida
|
|
LFI1238
|
Wild type, isolated from Atlantic cod
|
[11]
|
|
ΔlitR
|
LFI1238 containing an in-frame deletion of litR
|
[10]
|
|
ΔainS
|
LFI1238 containing an in-frame deletion of ainS
|
This study
|
|
ΔainS/luxI ˉ
|
ΔainS with insertional disruption of luxI; Cmr
|
This study
|
|
luxI ˉ
|
LFI1238 with insertional disruption of luxI; Cmr
|
This study
|
|
luxR1ˉ
|
LFI1238 with insertional disruption of luxR1 gene, Ermr
|
This study
|
|
luxR2ˉ
|
LFI1238 with insertional disruption of luxR2 gene, Ermr
|
This study
|
E. coli
|
|
S17-1
|
Donor strain for conjugation, λ-pir
|
[42]
|
|
DH5αλ pir
|
Donor strain for conjugation
|
[45]
|
|
DH5α
|
Helper strain for conjugation
|
Invitrogen
|
Plasmids
|
|
pDM4
|
Cmr; suicide vector with an R6K origin (λ-pir requiring) and sacBR
|
[43]
|
|
pNQ705
|
Cmr; suicide vector with an R6K origin (λ-pir requiring)
|
[43]
|
|
pEVS122
|
Ermr; suicide vector with an R6Kγ oriV, oriT
RP4
, lacZα,cosN, loxP, incD
|
[45]
|
|
pEVS104
|
Helper plasmid
|
[44]
|
|
pDM4ΔainS
|
pDM4 containing a fragment of ainS harbouring an internal deletion
|
This study
|
|
pNQ705luxI
|
pNQ705 containing a 245 bp fragment of luxI
|
This study
|
|
pEVSluxR1
|
pEVS122 containing a 224 bp fragment of luxR1
|
This study
|
|
pEVSluxR2
|
pEVS122 containing a 280 bp fragment of luxR2
|
This study
|