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Archived Comments for: Extracellular ATP inhibits twitching motility-mediated biofilm expansion by Pseudomonas aeruginosa

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  1. Missing reference in BMC Microbiology 2015, 15:55

    Thomas Wood, Pennsylvania State University

    7 July 2015

    I am surprised that this article, where a hydrolysis product of ATP was found to reduce P. aeruginosa twitching, failed to reference our earlier work. We showed three years earlier (see Microbial Biotechnol. 5: 560–572, 2012, doi doi:10.1111/j.1751-7915.2012.00338.x, “Interkingdom adenosine signal reduces Pseudomonas aeruginosa pathogenicity”) that the hydrolysis product of ATP, adenosine, reduces P. aeruginosa. swarming and biofilm formation (note that ATP becomes adenosine in the GI tract).  This key finding should have been referenced in this manuscript as it is directly related to P. aeruginosa motility (swarming) and ATP signaling with this strain and both manuscripts show extracellular ATP/adenosine reduces biofilm formation with the identical strain. Our report was the first manuscript to link ATP (i.e., adenosine from ATP) with a reduction in biofilm formation of this pathogen.  We also showed that the addition of adenosine nearly abolished swarming by preventing production of rhamnolipids, and that adenosine reduces production of the virulence factors pyocyanin, elastase, extracellular polysaccharide, siderophores and the Pseudomonas quinolone signal which led to reduced virulence with Caenorhabditis elegans. A whole-transcriptome analysis was also conducted which revealed that adenosine addition represses genes similar to an iron-replete condition. Therefore, we showed three years earlier that adenosine decreases P. aeruginosa pathogenicity as an interkingdom signal by causing genes related to iron acquisition to be repressed.

    Competing interests

    None declared