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Figure 3 | BMC Microbiology

Figure 3

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

Figure 3

Carbon sources and medium selection for the high-level production of rMP1102 at the shake flask level. A, B: Effects of different carbon sources on the total protein levels and antimicrobial activities of the fermentation supernatants of Pichia pastoris GAPMP1102; C: Total protein levels of the fermentation supernatants of Pichia pastoris GAPMP1102 in the different media; D Tricine-SDS-PAGE analysis of fermentation supernatant of Pichia pastoris GAPMP1102 in Med-1. Lane M: a total of 5 μl of protein molecular weight marker (from top to bottom: 40, 25, 15, 10, 4.6 and 1.7 kDa). Lanes 1–6: 10 μl of rMP1102 fermentation supernatants taken at 0, 24, 48, 72, 96, and 120 h of induction, respectively. The arrow indicates rMP1102. Each data point is the average of three replicates, and the error bars represent the standard deviation. Different lowercase letters (a, ab, b, c) above the bar indicate significant differences between groups (P < 0.05).

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BMC Microbiology

ISSN: 1471-2180

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