Figure 4

The deletion of FaTBCA increased sensitivity to low temperatures. A. Mycelial plugs of the wild-type GJ33, ΔFaTBCA-3 and ΔFaTBCA-3C were inoculated on PDA and incubated at 4, 10, 15, 20 and 25°C for 3.5 days. B. Inhibition percentages of mycelial growth of GJ33, ΔFaTBCA-3 and ΔFaTBCA-3C at 4, 10, 15 and 20°C. After incubation for 3.5 days, colony diameter in each plate was measured in two perpendicular directions with the original mycelial plug diameter (5 mm) subtracted from each measurement. For each plate, the average of the colony diameters was used for calculating the percentage of growth inhibition. The mycelium growth of each strain at 25°C was arbitrarily set as control. Line bars in each column denote standard errors of three experiments. * = significant difference for each temperature at a 95% coincidence interval by performing a t test. C. The mutant ΔFaTBCA-3 was unable to grow at 4°C after incubation for 7 or 21 days.