Figure 2

Northern blotting analyses of transcripts containing rocG , sivA , and rocA. RNAs were prepared from 168 (lane 1), 1A95 (degU32, lane 2), TM015 (degU32::cat, lane 3), and TM016 (degU32 rocR::kan, lane 4) cells for northern blotting analyses using probes for rocG (A), sivA (B), and rocA (C) transcripts under low-stringency conditions. (D) RNAs were prepared from 1A95 (degU32, lane 1), TM016 (degU32 rocR::kan, lane 2), TM017 (sivA::pMutin2, lane 3), and TM018 (degU32 sivA::pMutin2, lane 4) cells for northern blotting analyses using probes for rocG transcripts under high-stringency conditions; rRNAs (23S and 16S) on membranes were visualized as a loading control using methylene blue staining. Gene organization of the rocG locus is shown at the bottom.