Expression of TJ proteins in piglets following oral administration with
I5007. Four-day-old piglets were orally administrated with 6 × 109 CFU of L. reuteri I5007 per day for 14 days. Subsequently, proteins were isolated from the intestinal tissues of piglets and then the expression of claudin-1, occludin and ZO-1 was measured using immunoblotting. (A) Protein levels of occludin and ZO-1 in jejunal tissue lysates were induced by L. reuteri I5007. (B)
L. reuteri I5007 increased the expression of claudin-1, occludin and ZO-1 in the ileum. Data are represented as mean ± SEM, n = 6. *P < 0.05 compared with control piglets.