Cathepsins contribute to the anti-
effects of IL-32γ. A) THP-1 cells were infected with MTB alone, MTB + IL-32γ (50 ng/mL), or pre-incubated with combined cathepsin B and D inhibitors or with cathepsin L inhibitor for one hour and then incubated with MTB + IL-32γ. Intracellular CFU were quantified in one hour, two days, and four days after infection. B) TUNEL positive cells were quantified two days after MTB infection ± IL-32γ (50 ng/mL) with and without cathepsin B/D inhibitors or cathepsin L inhibitor. Data shown are the mean ± SEM of four independent experiments. *p < 0.05, **p < 0.01.