Figure 2
From: CrdR function in a curdlan-producing Agrobacterium sp. ATCC31749 strain
Confirmation of the ATCC31749 crdR mutant by PCR amplification. (A, B) crdR was replaced without and with gentamicin resistance gene (Gm) in ATCC31749; (C) PCR amplification. CM: 2Kb DNA ladder, C1, C3, C5: template of genomic DNA of wild-type ATCC31749, while C2, C4, C6: template of genomic DNA of ATCC31749ΔcrdR candidate were used respectively. C2: amplification of the Gm gene; C4: amplification of a fused fragment of the crdR upstream flanking region and the 5’-end of the Gm gene; C6: amplification of the fused fragment of 3’-end of the Gm and the crdR downstream flanking region.