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Figure 4 | BMC Microbiology

Figure 4

From: Identification of ferredoxin II as a major calcium binding protein in the nitrogen-fixing symbiotic bacterium Mesorhizobium loti

Figure 4

Identification of M . loti Ca 2+ binding proteins by 45 Ca 2+ -ligand overlay. Proteins were dot-blotted onto nitrocellulose and then incubated with 45CaCl2 in a calcium overlay assay [44]. 45Ca-labeled proteins were visualized by autoradiography (7-d exposure). 1, equine cytochrome c, used as negative control (5 μg); 2, purified M. loti 20 kDa Stains-all blue-staining protein (5 μg); 3, rabbit calsequestrin, used as positive control (1 μg).

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