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Figure 4 | BMC Microbiology

Figure 4

From: A new suite of tnaA mutants suggests that Escherichia coli tryptophanase is regulated by intracellular sequestration and by occlusion of its active site

Figure 4

The polar localizations of TnaA and D1D3 are not artifactual. A. D1D3-sfGFP foci do not overlap with inclusion bodies in E. coli GL730 cells coexpressing D1D3-sfGFP and IbpA-mCherry, which binds to protein inclusion bodies. The sfGFP image (left) and the mCherry overlay image (right) are shown. B. Plasmid-borne untagged TnaA (from plasmid pTnaA) competes with chromosomal D1D3-sfGFP for polar localization. D1D3-sfGFP (in strain GL726) was localized at cell poles when TnaA was not induced (left), but became diffuse when TnaA was induced (right). C. TnaA-mGFPmut3 (left) and D1D3-mGFPmut3 (right) form single foci at the cell poles in strains GL676 and GL734, respectively.

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BMC Microbiology

ISSN: 1471-2180

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