Figure 2

Influence of SEB on CD1d cell surface expression: Fluorescence-based CD1d reporting: 1 x 10 ⁶ cells/ml RPTECs were exposed to 100 μg/ml SEB for 1 h, washed and then probed using the mouse anti-CD1d antibody coupled to Alexa 594-conjugated goat anti-mouse (Red spots). This image is designated as (+) SEB. The (−) SEB image was obtained from the cells treated with the same method; however, SEB was replaced with PBS. All images are at 60X magnification captured with a fluorescent microscope equipped with a digital camera (Olympus Optical Company). The red spots represent the CD1d expressed on the cell surface.