Figure 1

Schematic diagram of csrA region in chromosome of GY448 and CsrA in Y. enterocolitica and E. coli . The location and orientation of the orf is indicated by the thick black arrow. The insertion location of the transposon with the kanamycin resistance cassette (Km) is shown above. The downward arrow from this location indicates the site of the mutation within codon 29 of the protein encoded by the Y. enterocolitica orf, which is represented by the black rectangle. The grey rectangle represents CsrA from E. coli. The dashed lines between these three components indicate alignment between the Y. enterocolitica orf, the Y. enterocolitica protein and the E. coli protein. The stars represent regions essential for dimerization. The numbers represent amino acid position. The small grey shaded region represents non-homology of the Y. enterocolitica protein with the E. coli protein. The location of the transposon insertion results in a C-terminal truncation that excludes one of the critical regions essential for dimerization.