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Table 1 Plasmids used for P. xanthii electroporation and their main features

From: Transient transformation of Podosphaera xanthii by electroporation of conidia

Plasmid designation Size (bp) Resistance and/or reporter genes Reference
pAN7-1 6756 E. coli hygromycin B phosphotransferase gene (hph), under the control of A. nidulans gpd (glyceraldehyde-3-phosphate dehydrogenase) promoter [33]
pGPDGFP 6400 Green fluorescent protein gene (gfp) fused to the gpd promoter and the tryptophan synthetase (trpC) terminator from A. nidulans [34]
pIGPAPA 5910 E. coli hph under the control of A. nidulans trpC promoter/gfp under the control of IsL (isocitrate lyase) promoter from N. crassa [35]
pCPXHY1eGFP 9100 E.coli hph under the control of A. nidulans trpC promoter/Enhanced green fluorescent protein gene (egfp) under the control of A. nidulans gpd promoter [32]
pCPXBteGFP 11630 Carbendazim resistant β-tubulin gene (TUB2) from P. xanthii under the control of its own promoter/egfp under the control of A. nidulans gpd promoter This study