Figure 7
From: Transient transformation of Podosphaera xanthii by electroporation of conidia
Western blot analysis of EGFP of P. xanthii transformants obtained with pCPXBteGFP. The total protein content from potential transformants and untransformed colonies was TCA-precipitated, separated by SDS-PAGE, blotted on PVDF membranes and immunoprobed with anti-GFP antibodies (1:1000). The size of the major cross-reacting band is indicated on the right. The same amount of protein (1 μg ml−1) was loaded per lane. M, molecular size marker Spectra Multicolor Broad Range Protein Ladder (Fermentas).