Western blot analysis of EGFP of
transformants obtained with pCPXBteGFP. The total protein content from potential transformants and untransformed colonies was TCA-precipitated, separated by SDS-PAGE, blotted on PVDF membranes and immunoprobed with anti-GFP antibodies (1:1000). The size of the major cross-reacting band is indicated on the right. The same amount of protein (1 μg ml−1) was loaded per lane. M, molecular size marker Spectra Multicolor Broad Range Protein Ladder (Fermentas).