Molecular analysis of
transformants obtained after electro-transformation with plasmid pCPXHY1eGFP. Genomic DNA from potential transformants was subjected to PCR amplification of the egfp gene using the primer pair GFP-F and GFP-R. The size of the expected PCR product is indicated on the left. Lanes are: 1, the plasmid pCPXHY1eGFP; 2, DNA from an untransformed colony; 3–8, potential hygromycin B-resistant and fluorescent transformants. M, molecular size marker 1 kb ladder.