Figure 2
From: Transient transformation of Podosphaera xanthii by electroporation of conidia
Transformation of P. xanthii conidia with plasmid pCPXHY1eGFP. (A) Untransformed colonies grown normally on non-treated zucchini cotyledons. (B) Transformants of P. xanthii obtained with pCPXHY1eGFP grown onto hygromycin B treated cotyledons. Note the reduced colony size and the damage on the host tissues caused by hygromycin B. (C-D) CLSM analysis of P. xanthii transformants. (C) An untransformed colony with no GFP fluorescence signal. (D) A transformant colony showing GFP fluorescence in the peripheral hyphae, whereas conidia did not show fluorescence (arrow). Pictures were taken 15 d after electroporation of conidia. Scale bars 100 μm.