The near-quantitative sampling of genomic DNA from various food-borne Eubacteria

BMC Microbiology

Table 1 Colony forming unit-normalized 16S r DNA copy number for various extraction protocols associated with three biological replicates of Brochothrix thermosphacta

	Brochothrix copies 16SrRNA “gene” per CFU
Extraction methods	Culture 1	Culture 2	Culture 3	Mean	Stdev
Fast ID	0.265	0.353	0.349	0.322	0.0497	a
Boiling Water	0.222	1.05	0.693	0.655	0.415	a
Trizol	0.484	1.74	0.643	0.956	0.684	a
DNEasy	0.602	1.72	1.28	1.20	0.561	a
PrepMan	0.916	2.67	2.04	1.88	0.889	a
Genscript	8.67	10.9	8.72	9.43	1.27	a
BeadBug	18.7	21.6	25.8	22.0	3.57	ab
Agencourt	143	189	91.4	141	48.8	bc
QuikExtract (Ampure)	329	73.6	248	217	131	c
High Pure	596	544	579	573	26.9	d
Omnilyse	918	956	927	934	19.9	e
		CFU mL⁻¹
	8.62 × 10⁶	6.92 × 10⁶	7.92 × 10⁶
	±5.66%	±4.46%	±7.44%

Any two means reported with different letters are significantly different at the P = 5 × 10^-2 level.

ISSN: 1471-2180