Figure 2

Accumulation periplasmic PG fragments or protein correlates with increased vesiculation without alteration of Lpp crosslinking. (A) Relative fold crosslinked Lpp in cultures of the indicated strains grown in LB to an OD₆₀₀ of ~ 0.4 at 37°C was determined as described in Figure 1B. (B) Relative fold of free Lpp in cultures of the indicated strains grown overnight in LB at 37°C was determined as described in Figure 1C. (C) Relative fold OMV production in cultures of the indicated strains grown in LB overnight at 37°C was determined as described in Figure 1A. Error bars indicate SEM. p values refer to comparisons with WT unless indicated by a bracket. *, p ≤ 0.05; NS, p > 0.05; n ≥ 3.