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Table 2 PHA production of different phaC genes in recombinant C. necator PHBˉ4 a

From: Whole genome amplification approach reveals novel polyhydroxyalkanoate synthases (PhaCs) from Japan Trench and Nankai Trough seawater

Sample name

Dry cell weight (g/L)

PHA content (wt %)

PHA composition

C. necator H16 (Wild type)

4.1 ± 0.5

70 ± 5

P(3HB)

C. necator PHBˉ4(PHB-negative mutant)

1.2 ± 0

n/d

n/d

I-GG1

0.9 ± 0

n/d

n/d

I-GG12

0.9 ± 0

n/d

n/d

I-GG18

2.6 ± 0.1

60 ± 8

P(3HB)

I-GG18 (without promoter)

0.9 ± 0

n/d

n/d

  1. aCells were cultivated in MM medium at 30°C, 200 rpm for 48 hours with 20 gL−1 of fructose as the sole carbon source.
  2. n/d = not detected.
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BMC Microbiology

ISSN: 1471-2180

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