Figure 1

Protein neighbor-joining phylogenetic tree of PHA synthases (PhaCs). All putative PhaCs obtained from culture-independent studies, published [20]-[22],[47] and unpublished were subjected to phylogenetic classification. Colored labels indicate the presence of known PHA producers in the same genus. Red stars indicate novel or uncharacterized putative PhaCs. Orange color boxes represent the complete CDS of PhaCs used for PHA production in this study. The scale represents the number of amino acid substitutions per site. Bootstrapping values less than 50 are not shown in the tree. Accession numbers for the PhaC sequences are indicated after the sequence name and can also be found in Additional file 4: Table S4. PhaC of Bacillus megaterium was used as an outgroup for the phylogenetic tree.