(n = 71)
|
Blood culture
|
Nested multiplex qPCR
|
SeptiFast (Roche)
|
FISH
|
βactin gene
|
---|
Gram positive
|
Gram negative
|
Yeast
|
Filamentous fungi
|
---|
1
| | | | | | | |
+
|
2
| | | | | | | |
+
|
3
|
S. warneri
| | | | | | |
+
|
4
| | | | | | | |
+
|
5
|
S. epidermidis
|
+
| | | |
CoNS
|
+
|
+
|
6
| | |
+
| | | | |
+
|
7
|
S. haemolyticus
|
+
| | | | |
+
|
+
|
8
| | | | | | | |
+
|
9
|
S. epidermidis
|
+
|
+
| | |
CoNS
|
+
|
+
|
10
| | | | | | | |
+
|
11
| | |
+
| | | | |
+
|
12
| | |
+
| | | | |
+
|
13
|
E. cloacae
| |
+
| | |
E. cloacae/aerogrenes
|
+
|
+
|
14
|
P. aeruginosa
| |
+
| | | |
+
|
+
|
15
|
S. haemolyticus
|
+
| | | |
Staphylococcus. spp
|
+
|
+
|
16
| | |
+
| | | | |
+
|
17
| | |
+
| | | | |
+
|
18
|
S. hominis
|
+
| | | | |
+
|
+
|
19
| | |
+
| | | | |
+
|
20
| | | | | | | |
+
|
21
| | |
+
| | |
E. cloacae/aerogrenes
| |
+
|
22
| |
+
| | | | | |
+
|
23
| |
+
| | | | | |
+
|
24
| | |
+
| | | | |
+
|
25
| | |
+
| | |
E. cloacae/aerogrenes
|
+
|
+
|
26
| | | | | | | |
+
|
27
| | |
+
| | | | |
+
|
28
|
S. haemolyticus
|
+
| | | |
CoNS
|
+
|
+
|
29
| | | | | | | |
+
|
30
| | |
+
| | |
E. cloacae/aerogrenes
| |
+
|
31
| | |
+
| | | | |
+
|
32
| | |
+
| | |
K. pneumoniae/oxytoca
| |
+
|
33
| | |
+
| | | | |
+
|
34
|
E. faecium
|
+
|
+
| | |
E. faecium
|
+
|
+
|
35
|
S. mitis
|
+
|
+
| | |
Streptococcus. spp
|
+
|
+
|
36
|
S. hominis
| |
+
| | | |
+
|
+
|
37
|
S. haemolyticus
| | | | | | |
+
|
38
| | |
+
| | | | |
+
|
39
|
P. aeruginosa
| |
+
| | |
P. aeruginosa
|
+
|
+
|
40
| | |
+
| | | | |
+
|
41
| | |
+
| | |
A. fumigatus
| |
+
|
42
| | |
+
| | | | |
+
|
43
| | |
+
| | | | |
+
|
44
|
S. epidermidis
| | | | | | |
+
|
45
| | |
+
| | | | |
+
|
46
| | |
+
| | | | |
+
|
47
|
C. albicans
| | | | |
C. albicans
| |
+
|
48
| | | | | | | |
+
|
49
| | |
+
| | |
K. pneumoniae/oxytoca
| |
+
|
50
|
E. cloacae
| | | | | | |
+
|
51
| | | | | | | |
+
|
52
| | | | | | | |
+
|
53
|
S. hominis
|
+
|
+
| | | | |
+
|
54
| |
+
|
+
| | |
S. aureus
| |
+
|
55
| | | | | | | |
+
|
56
| | |
+
| | | | |
+
|
57
| |
+
|
+
| | | | |
+
|
58
| |
+
|
+
| | | | |
+
|
59
|
K. pneumoniae
|
+
|
+
| | | | |
+
|
60
|
S. epidermidis
|
+
|
+
| | | |
+
|
+
|
61
| | |
+
| | | | |
+
|
62
|
P. acnes
|
+
|
+
| | | | |
+
|
63
| |
+
|
+
| | |
E. cloacae/aerogenes
|
+
|
+
|
64
| |
+
|
+
| | | |
+
|
+
|
65
|
P. aeruginosa and S. hominis
| | | | | | |
+
|
66
|
S. epidermidis
|
+
|
+
| | | |
+
|
+
|
67
|
K. pneumoniae
|
+
|
+
| | |
K. pneumoniae/oxytoca
|
+
|
+
|
68
|
S. aureus
|
+
|
+
| | | |
+
|
+
|
69
|
S. epidermidis
|
+
|
+
| | | |
+
|
+
|
70
| |
+
|
+
| | | |
+
|
+
|
71
| | |
+
| | | | |
+
|
positive
|
26
*
|
24
|
46
|
0
|
0
|
18
*
|
21
*
|
71
|
51
|
%
|
36.6
|
33.8
|
64.8
|
0
|
0
|
25.3
|
29.6
|
100
|
71.8
|
Sensitivity [CFU/ml]
|
1x10
1[1]
|
1.1x10
1[13]
|
1.3x10
1[13]
|
8.5x10
1[13]
|
3.7x10
1[13]
|
3x10
0
– 3x10
1[28]
|
6x10
3[14]
|
-
|
- CoNS – coagulase negative Staphylococcus.
- CFU = Colony Forming Unit.
- „+” – positive result.
- „*” – statistically significant differences in comparison with the nested, multiplex, qPCR (there was no significance between the other methods). Cochran’s Q test; p < 0.0001.
- The bold data - summary of results.