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Table 1 Comparison of the results obtained from blood of patients with clinical symptoms of sepsis by the method of blood culture, the nested multiplex qPCR, FISH and SeptiFast (Roche) methods and the internal inhibition control for the β-actin gene

From: Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis

(n = 71) Blood culture Nested multiplex qPCR SeptiFast (Roche) FISH βactin gene
Gram positive Gram negative Yeast Filamentous fungi
1         +
2         +
3 S. warneri        +
4         +
5 S. epidermidis +     CoNS + +
6    +      +
7 S. haemolyticus +      + +
8         +
9 S. epidermidis + +    CoNS + +
10         +
11    +      +
12    +      +
13 E. cloacae   +    E. cloacae/aerogrenes + +
14 P. aeruginosa   +     + +
15 S. haemolyticus +     Staphylococcus. spp + +
16    +      +
17    +      +
18 S. hominis +      + +
19    +      +
20         +
21    +    E. cloacae/aerogrenes   +
22   +       +
23   +       +
24    +      +
25    +    E. cloacae/aerogrenes + +
26         +
27    +      +
28 S. haemolyticus +     CoNS + +
29         +
30    +    E. cloacae/aerogrenes   +
31    +      +
32    +    K. pneumoniae/oxytoca   +
33    +      +
34 E. faecium + +    E. faecium + +
35 S. mitis + +    Streptococcus. spp + +
36 S. hominis   +     + +
37 S. haemolyticus        +
38    +      +
39 P. aeruginosa   +    P. aeruginosa + +
40    +      +
41    +    A. fumigatus   +
42    +      +
43    +      +
44 S. epidermidis        +
45    +      +
46    +      +
47 C. albicans      C. albicans   +
48         +
49    +    K. pneumoniae/oxytoca   +
50 E. cloacae        +
51         +
52         +
53 S. hominis + +      +
54   + +    S. aureus   +
55         +
56    +      +
57   + +      +
58   + +      +
59 K. pneumoniae + +      +
60 S. epidermidis + +     + +
61    +      +
62 P. acnes + +      +
63   + +    E. cloacae/aerogenes + +
64   + +     + +
65 P. aeruginosa and S. hominis        +
66 S. epidermidis + +     + +
67 K. pneumoniae + +    K. pneumoniae/oxytoca + +
68 S. aureus + +     + +
69 S. epidermidis + +     + +
70   + +     + +
71    +      +
positive 26 * 24 46 0 0 18 * 21 * 71
51
% 36.6 33.8 64.8 0 0 25.3 29.6 100
71.8
Sensitivity [CFU/ml] 1x10 1[1] 1.1x10 1[13] 1.3x10 1[13] 8.5x10 1[13] 3.7x10 1[13] 3x10 0 – 3x10 1[28] 6x10 3[14] -
  1. CoNS – coagulase negative Staphylococcus.
  2. CFU = Colony Forming Unit.
  3. +” – positive result.
  4. „*” – statistically significant differences in comparison with the nested, multiplex, qPCR (there was no significance between the other methods). Cochran’s Q test; p < 0.0001.
  5. The bold data - summary of results.
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