BMC Microbiology

Table 1 Comparison of the results obtained from blood of patients with clinical symptoms of sepsis by the method of blood culture, the nested multiplex qPCR, FISH and SeptiFast (Roche) methods and the internal inhibition control for the β-actin gene

From: Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis

(n = 71)	Blood culture	Nested multiplex qPCR				SeptiFast (Roche)	FISH	βactin gene
(n = 71)	Blood culture	Gram positive	Gram negative	Yeast	Filamentous fungi	SeptiFast (Roche)	FISH	βactin gene
1								+
2								+
3	S. warneri							+
4								+
5	S. epidermidis	+				CoNS	+	+
6			+					+
7	S. haemolyticus	+					+	+
8								+
9	S. epidermidis	+	+			CoNS	+	+
10								+
11			+					+
12			+					+
13	E. cloacae		+			E. cloacae/aerogrenes	+	+
14	P. aeruginosa		+				+	+
15	S. haemolyticus	+				Staphylococcus. spp	+	+
16			+					+
17			+					+
18	S. hominis	+					+	+
19			+					+
20								+
21			+			E. cloacae/aerogrenes		+
22		+						+
23		+						+
24			+					+
25			+			E. cloacae/aerogrenes	+	+
26								+
27			+					+
28	S. haemolyticus	+				CoNS	+	+
29								+
30			+			E. cloacae/aerogrenes		+
31			+					+
32			+			K. pneumoniae/oxytoca		+
33			+					+
34	E. faecium	+	+			E. faecium	+	+
35	S. mitis	+	+			Streptococcus. spp	+	+
36	S. hominis		+				+	+
37	S. haemolyticus							+
38			+					+
39	P. aeruginosa		+			P. aeruginosa	+	+
40			+					+
41			+			A. fumigatus		+
42			+					+
43			+					+
44	S. epidermidis							+
45			+					+
46			+					+
47	C. albicans					C. albicans		+
48								+
49			+			K. pneumoniae/oxytoca		+
50	E. cloacae							+
51								+
52								+
53	S. hominis	+	+					+
54		+	+			S. aureus		+
55								+
56			+					+
57		+	+					+
58		+	+					+
59	K. pneumoniae	+	+					+
60	S. epidermidis	+	+				+	+
61			+					+
62	P. acnes	+	+					+
63		+	+			E. cloacae/aerogenes	+	+
64		+	+				+	+
65	P. aeruginosa and S. hominis							+
66	S. epidermidis	+	+				+	+
67	K. pneumoniae	+	+			K. pneumoniae/oxytoca	+	+
68	S. aureus	+	+				+	+
69	S. epidermidis	+	+				+	+
70		+	+				+	+
71			+					+
positive	26 *	24	46	0	0	18 *	21 *	71
positive	26 *	51				18 *	21 *	71
%	36.6	33.8	64.8	0	0	25.3	29.6	100
%	36.6	71.8				25.3	29.6	100
Sensitivity [CFU/ml]	1x10 ¹[1]	1.1x10 ¹[13]	1.3x10 ¹[13]	8.5x10 ¹[13]	3.7x10 ¹[13]	3x10 ⁰ – 3x10 ¹[28]	6x10 ³[14]	-

CoNS – coagulase negative Staphylococcus.
CFU = Colony Forming Unit.
„+” – positive result.
„*” – statistically significant differences in comparison with the nested, multiplex, qPCR (there was no significance between the other methods). Cochran’s Q test; p < 0.0001.
The bold data - summary of results.

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