Figure 7
From: Plasminogen activation by staphylokinase enhances local spreading of S. aureus in skin infections
Gelatinase activation by SAK in skin tissue. A. Gelatin zymogram showing pro-MMP-2 and active MMP-2 in murine skin and subcutaneous tissue protein extract after incubation with staphylokinase (SAK) and human plasminogen (huPlg). Activation with APMA, a chemical MMP-activator, was used as a positive control. Quantitation of MMP-2 activation (active/total), data are mean ± SD from 3 independent experiments. *denotes P < 0.05. B. Gelatin zymogram showing pro-MMP-2 and active MMP-2 in day 3 lesional skin samples. A sample from normal skin and an APMA-activated normal skin extract are included as controls. Quantitation of MMP-2 activation (active/total), data are mean ± SD from 3 independent experiments. P for trend <0.05. C. MMP-9 activation in day 3 lesional skin samples, assessed by Western blot. A sample from normal skin is included as control. Quantitation of MMP-9 activation (active/total), data are mean ± SD from 3 independent experiments. P for trend <0.05.