Figure 5

Hyper-biofilm forming evolved strains of the fuzzy and wrinkled morphotypes have mutations in the master biofilm regulator sinR. Schematic of the SinR protein and relevant sequence. (A) Red letters and blue boxes represent amino acids affected in the evolved strains. The numbers above or below the sequence indicate the amino acid number within the full-length protein. (B) The sinR mutations identified in ST1, SH3, and SH4 were transferred into the ancestral strain (from left to right, R1, R3, R4). All strains were plated on LB +1% agar, and colonies arising from single cells were photographed after 24 hours. All images were taken at the same scale. Scale bar, 250 mm.