Figure 2

Transposon insertion density across a selected region of the RLV3841 genome. growth-defective, neutral, and growth-advantage regions of the RLV3841 genome. Analyzing the total number of insertions mapped to each potential mariner insertion site using the Tn-HMM python module [10] allows each gene to be placed in one of four phenotypic classes: ES = essential, GD = growth defect, NE = neutral, GA = growth advantage. In this experiment the phenotype refers to the ability to grow on TY agar. Data visualization was obtained using Integrative Genomics Viewer software [54]. Please refer to the Materials and Methods section for more detail on the process of assigning phenotypic classifications.