Figure 3

Co-purification of FpvR _1–89 with either His ₆ -FpvI or His ₆ -PvdS from E. coli . Soluble protein was obtained from E. coli MC1061 (DE3) co-expressing either His₆-FpvI and FpvR_1-89 or His₆-PvdS and FpvR_1–89. Protein was purified by nickel affinity chromatography via the His₆-tags on PvdS and FpvI and analyzed by SDS-PAGE (top panels) and Western blotting (lower panels) using anti-FpvI, anti-PvdS or anti-FpvR antibodies as shown. (A) Co-purification of FpvR_1–89 with His₆-FpvI; (B) co-purification of FpvR_1-89 with His₆-PvdS. The positions of molecular weight markers are shown.