Figure 1

Co-purification of PvdS with FpvR _1–89 –TAP from P. aeruginosa . Soluble protein was prepared from P. aeruginosa PAO1 fpvR expressing plasmid-borne (pUCP23) or chromosomally-integrated (ctx) FpvR_1–89 fused to a C-terminal TAP tag. Protein was purified using the TAP protocol and the purified protein analyzed by Western blotting for FpvR_1–89-CBP or PvdS. (A) anti-CBP; (B) anti-FpvR; (C) anti-PvdS. A mock purification was carried out with P. aeruginosa PAO1 fpvR carrying pUCP23 without the fpvR _1–89 -TAP fusion as a negative control for the TAP tag purification procedure. The positions of molecular weight markers are shown.