Figure 5

Abundance of mRNA encoding HD-GYP domain proteins in V. Cholerae biofilms. qRT-PCR was used to measure the transcript abundance for each HD-GYP mRNA, the reference housekeeping gene rpoB, and the biofilm-induced control gene vpsT. The relative differences in transcript abundance between biofilm and planktonic V. cholerae cells were determined as described in the Methods. *P <0.05 by unpaired t-test comparing biofilm to planktonic values, indicating a significant change in transcript abundance in biofilm relative to planktonic cells.