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Figure 1 | BMC Microbiology

Figure 1

From: Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection of novel avian influenza A (H7N9) virus

Figure 1

Sensitivity and specificity of the H7/N9 -specific RT-LAMP assay. Amplification curves of the H7/N9-special RT-LAMP were performed with 10-fold serial dilutions of viral RNA (from 200 to 0.002 PFU per reaction). Specificities of H7 and N9 RT-LAMP were tested by using direct visual detection of RT-LAMP with RNA extracted from viral culture of H1N1, H3N2, H5N1, PIV3, H9N2, RSV, HAdV-3 and H7N9. (A) Detection limit of H7-specific RT-LAMP was 0.2 PFU per reaction. (B) The color changes under natural or UV light were only observed in the tube of H7N9 viral RNA by using H7-specific RT-LAMP assay. (C) Detection Limit of N9-specific RT-LAMP was 0.2 PFU per reaction. (D) The color changes were observed for N9-specific RT-LAMP reaction with H7N9 viral RNA. No color change was seen in RT-LAMP with other subtype of influenza viral RNA and other three respiratory viruses under natural or UV light.

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BMC Microbiology

ISSN: 1471-2180

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